FIG. 5.

GFP expression from the minigenome system by combinations of rubulavirus NP, P, L, and V proteins. (A) Effect of heterogeneous sets containing NP, P, and L plasmids derived from hPIV2 and PIV5. BSR T7/5 cells were transfected with pPIV2-GFP and all possible combinations of hPIV2 and PIV5 NP, P, and L expression plasmids. −, No plasmid was included as a negative control. Repression of GFP expression from minigenome system of homologous sets of hPIV2 (B) or PIV5 (C) by rubulavirus V proteins. After 48 h, the cells were assayed by Western blotting with anti-NP, P/V, PIV5-V, L, and GFP antibodies.