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. 2008 Apr 16;82(13):6130–6138. doi: 10.1128/JVI.02635-07

FIG. 7.

FIG. 7.

Analysis of interactions between L and V proteins by immunoprecipitation. (A) BSR T7/5 cells were transfected with plasmids expressing hPIV2 L and mutant hPIV2 V proteins. At 48 hpt, the cell extracts were either analyzed directly by Western blot analysis (anti-L, upper panel; anti-V, middle panel) or immunoprecipitated with anti-V before Western blot analysis (anti-L, lower panel). (B and C) BSR T7/5 cells were transfected with plasmids expressing hPIV2 (B) or PIV5 (C) L and rubulavirus V proteins. At 48 hpt, the cell extracts were either analyzed directly by Western blot analysis (anti-L, upper panel; anti-V and PIV5-V, middle panel) or immunoprecipitated with anti-V (B) or PIV5-V (C) before Western blot analysis (anti-L, lower panel).