TABLE 1.

Oligonucleotide primers used in the present study

Region and application	Direction	Primer	Sequence	Positiona
NS5A-1b
RT and first PCR	Antisense	1a1b-ISV3-AS	5′-ACGCCTTCGCCTTCATCTCC-3′	7812-7793
First PCR	Sense	1a1b-ISV3-S	5′-CCCATCAACGCATACACCACG-3′	6510-6531
Second PCR and sequencing	Antisense	2a1b-ISV3-AS	5′-GGACATTGAGCAGCAKACGAC-3′	7595-7575
	Sense	2a1b-ISV3-S	5′-GACCCCTCYCAYATYACAGCAG-3′	6858-6879
NS5A-1a
RT and first PCR	Antisense	1a1a-ISV3-AS	5′-GACATTGAGCAGCACACGAC-3′	7609-7590
First PCR	Sense	1a1a-ISV3-S	5′-CCATTAAYGCCTACACCACG-3′	6523-6542
Second PCR and sequencing	Antisense	2a1a-ISV3-AS	5′-TACTGACCGTYGACCATGAC-3′	7569-7560
	Sense	2a1a-ISV3-S	5′-ATCCCTCCCATATAACAGCAG-3′	6871-6891
PePHD-1b
RT and first PCR	Antisense	1a1b-PePHD-AS	5′-CGCAGAAGAACACRAGRAAGGAG-3′	2627-2649
First PCR	Sense	1a1b-PePHD-S	5′-CCATACAGRCTYTGGCACTACC-3′	2163-2184
Second PCR	Antisense	2a1b-PePHD-AS	5′-ACRGACGCYGCATTGAG-3′	2605-2589
	Sense	2a1b-PePHD-S	5′-AAGGTYAGGATGTATGTGGG-3′	2211-2230

^aNucleotide positions are according to prototypes HCV-J (genotype 1b) and HCV-1 (genotype 1a).