FIG. 2.

Insertion of antigenic tags in both gK and UL20p does not adversely affect virus replication, cell-to-cell spread, or plaque formation. (A and B) Confluent Vero cell monolayers were infected with either parental wild-type KOS virus (A) or the isolated dual-epitope-tagged recombinant D3gKprtC/UL20FLAG virus (B) at an MOI of 0.001, and viral plaques were visualized by immunohistochemistry at 48 hpi. (C) Detection and characterization of the protC-tagged gK or FLAG-tagged UL20p expressed in D3gKprtC/UL203×FLAG virus-infected cells. Specific detection of either gK (left) or UL20p (right) was achieved using antibodies against prtC (gK) or 3× FLAG (UL20p).