FIG. 5.

(A) Schematic of UL20p membrane topology. The intracellular amino terminus (domain I) of UL20p that was cloned in frame with GST for bacterial expression is highlighted within the boxed region. (B) SDS-PAGE and Coomassie blue staining of crude cell lysates from bacterially expressed GST and GST fused with the amino terminus of UL20p. (C) SDS-PAGE and Coomassie blue staining of glutathione column-purified bacterially expressed GST and GST fused with the amino terminus of UL20p. (D) Immunoblot specific detection of either GST or GST fused with the amino terminus of UL20p using anti-GST antibody.