Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The amino terminus of UL20p directly interacts with gK in transfected and infected Vero cells. Infected cell lysates were directly loaded (no precipitation) (lane 3) or incubated with GST (lane 1) or the purified UL20 amino terminus fused with GST (lane 2) and pulled down with glutathione-conjugated agarose. Blots of SDS-PAGE-separated precipitates were probed with anti-GST, anti-FLAG (αUL20), anti-prtC (αgK), or αgB and αgD in combination in order to detect GST pull-down proteins.
