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. 2008 Apr 23;82(13):6310–6323. doi: 10.1128/JVI.00147-08

FIG. 7.

FIG. 7.

UL20p transport from ER to TGN is required for the coordinate transport of gK and UL20p to post-ER cellular membranes. (A and B) Vero cells were cotransfected with plasmids expressing gK and either UL20 specifying the ER-retained UL20p(KKSL) (B) (UL20 ER ret.) or the UL20p(KKSLAL) control protein (A) (UL20 ER ret. control). (A) Coexpression of UL20 ER ret. control protein (red) and gK (blue), showing gK and UL20p cotransport to the TGN (green) membranes. (B) Coexpression of UL20 ER ret. protein (red) with gK (blue), showing lack of UL20p and gK transport to TGN (green) membranes. (C to F) Vero cells were infected with viruses that expressed either an ER-retained UL20 (D, E, and F) (UL20 ER ret.) or UL20 ER ret. control proteins (C). (C) Infection with the virus specifying the wild-type like UL20 ER ret. control protein, showing accumulation of gK (red) on cell surfaces (blue) and Golgi compartments (green). (D) Infection with the virus expressing UL20 ER ret. protein exhibited retention of gK (red) in the ER (green) and not on cell surfaces (blue). (E) Infection with the virus specifying UL20 ER ret. control protein, showing that UL20 (red) was transported to neither cell surfaces (blue) nor Golgi compartments (green). (F) Infection with a virus specifying UL20 ER ret. protein (blue), showing retention of both the UL20 ER ret. protein (blue) and gK (red) in the ER and lack of UL20 and gK transport to the Golgi compartments (green). Magnification, ×63 (zoom, ×4).