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UL20 ER retention abrogates virus-induced cell-to-cell fusion. Vero cells were transfected with negative control plasmids (A1 and B1) or plasmids encoding wild-type (A2 and B2) or UL20p ER retention (A3 and B3) genes and then infected with either the Δ20gKsyn1 or the Δ20gBsyn3 virus. At 24 hpi, the ability of these plasmids to complement virus-induced cell fusion was determined by visualization of syncytia formation by fluorescence microscopy.
