FIG. 3.

Restriction specificities of primate TRIM5 proteins. (A) Amino acid alignment of primate TRIM5α proteins. Functional domains are indicated. The asterisk indicates the position of the amino acid difference with the published variants of gorTRIM5α. Numbers indicate the three residues that differ in the gorTRIM5α SPRY domain compared to huTRIM5α and cpzTRIM5α. (B) Expression of primate TRIM5 HA-tagged proteins in stable CHO-derived cell lines as determined by Western blot analysis using an anti-HA antibody (Covance). (C to H) Infection of CHO cells that were unmodified (white bars) or that stably expressed the indicated primate TRIM5 proteins (black bars) by wild-type and chimeric HIV-1 or NMLV or B-tropic murine leukemia virus (BMLV). Infected cells were enumerated by FACS analysis. The dose of each virus was selected to infect 15% to 30% of unmodified CHO cells.