FIG. 9.

Effect of UL46 on the expression of the IE ICP27 gene in lymphocytes. Jurkat T cells were either mock infected or infected with the indicated viruses at a multiplicity of infection (MOI) of 1, 10, or 100 PFU/cell. At 4 h postinfection, the accumulation of the IE protein ICP27 was assessed by Western blotting. (A) ICP27 and host β-actin were simultaneously detected with fluorescently labeled secondary antibodies using an Odyssey infared imager. (B) ICP27 signal intensities normalized to β-actin levels are displayed.