FIG. 2.

Transfection with siRNAs depletes the levels of eIF4H or eIF4B. HeLa cells were transfected with no siRNA, with siRNAs for GAPDH or eIF4B, or with three different siRNAs for eIF4H as indicated above the gel lanes. Cell lysates were prepared 48 h after transfection and assayed by Western blotting for eIF4B or eIF4H as indicated to the left of the gel. Each eIF4H-specific siRNA significantly reduced the level of eIF4H, but not eIF4B. The eIF4B siRNA depleted the level of eIF4B, but not eIF4H.