FIG. 6.
Intracellular distribution of K1 and PI3K. (A) HeLa cells were transfected with a K1WT expression plasmid. Forty-eight hours posttransfection, the cells were surface labeled with anti-FLAG-Cy3 to detect K1. After fixation and permeabilization, the cells were stained using an anti-p85 primary antibody, followed by a FITC-conjugated secondary antibody to detect the p85 subunit of PI3K. (B) Forty-eight hours posttransfection, K1WT-expressing HeLa cells were fixed, permeabilized, and stained with anti-FLAG-Cy3 and anti-p85 primary antibody, followed by a FITC-conjugated secondary antibody. The cells were analyzed by confocal microscopy.