FIG. 9.

Inhibition of clathrin-mediated endocytosis prevents K1 signaling. K1-expressing HeLa cells were treated with either amantadine or hypertonic sucrose medium to inhibit clathrin or were left untreated for 30 min prior to harvesting of the cells. The lysates were subjected to Western blot analysis. Equal amounts of proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with phosphospecific antibody to Akt S473 (top), stripped, and reprobed with antibody that recognized total Akt levels (bottom). No Tx, untreated cells.