FIG. 5.

IE1/LEF3/P143-associated structures exclude cellular chromatin. BmN cells were transfected with plasmids expressing IE1-GFP (A and B), LEF3-GFP (C and D), and H4-DsR (A and C), in conjunction with plasmids (+) or no plasmids (−) containing the indicated genes. The transfected cells were directly analyzed by confocal microscopy at 24 h posttransfection (A and C) or fixed at 24 h posttransfection and stained with a DNA-specific dye (PI) before the microscopic analysis (B and D). Lack of the ie1 gene caused a failure to express LEF3-GFP (data not shown). Arrows indicate IE1 foci. Bars, 10 μm.