FIG. 3.

Monocytes display diminished intracellular CA signal after infection with VSVg-pseudotyped HIV. Monocytes were infected with increasing viral inputs for 2 h, along with HeLaP4 cells and GM-CSF-differentiated macrophages. Loosely bound and noninternalized virion particles were removed by trypsin treatment. Cells were then permeabilized and examined for their CA content by flow cytometry using a specific anti-CA antibody. The figure presents data obtained with three to four different donors showing the percentage of CA⁺ cells (A) and the MFIs of positive cells at an MOI of 20, normalized to the value obtained in HeLaP4 cells (B). ***, P = 0,009 (Student t test).