In vitro BCD toxicities on various cell lines. GHOST-Hi5 cells, Caco-2 cells, and HeLa cells were exposed to medium containing 0, 2.5, 5, 10, or 20% BCD for 2 hours. The cells were trypsinized and counted by trypan blue exclusion. The dashed lines indicate the 50% toxic-dose concentration of BCD on each cell type. The points represent the averages of duplicate wells, and the error bars represent the standard deviations of duplicate wells. The graph is representative of two or three independent experiments.