FIG. 3.
(A) In vitro inhibition of HIV-1 and SIV infection by BCD. GHOST-Hi5 cells were infected with HIV-1LAI, HIV-1NFNSX, and SIVmac251 in medium containing no BCD or 5% BCD for 2 hours. The cells were washed with PBS, and the medium was replaced with new medium without BCD. The cells were incubated for 48 h, trypsinized, fixed, and analyzed for green fluorescent protein expression on a FACSCaliber. The graph is representative of three independent experiments. The error bars indicate standard deviations. (B) Replication kinetics of HIV-1 or SIV incubated in the presence or absence of BCD. Supernatants from GHOST-Hi5 cells infected with HIV-1LAI or SIVmac251 in the presence or absence of 5% BCD were added to CEMx174 cells. The cells were passaged for 3 weeks, and the supernatants were analyzed for p24 or p27 production by ELISA.