FIG. 4.
PPCM inhibits infection of human mucosal tissue. Ectocervical (squares, continuous line), endocervical (circles, dashed line), and penile (triangles, dotted line) explants were exposed to HIV-1BaL in the presence or absence of various concentrations of PPCM. Virus and PPCM were removed by extensive washing after 2 h. Following overnight incubation, explants were transferred to a new plate and cultured for 10 days. Fifty percent of the culture medium was replaced every 2 to 3 days, and culture supernatants were analyzed for p24 content by ELISA. Toxicity was assessed by an MTT assay following 24 h of exposure. Results are means ± standard errors of the means for three independent experiments (two for penile tissue) with different donors. Each condition was tested in triplicate.