FIG. 3.

Association of flaviviral nonstructural proteins with DRM fractions. (A) N18 cells were infected with JEV or DEN-2 (MOI = 10) for 18 h. The cells were lysed using 1% Triton X-100 in TNE buffer and then subjected to sucrose gradient ultracentrifugation. The floating bands were collected for Western blotting analysis as described in Materials and Methods. (B) N18 cells were infected with JEV (MOI = 10) for 16 h, and the cell lysates collected by use of 1% NP-40-LSB were subjected to sucrose gradient ultracentrifugation as described in Materials and Methods. The samples in each fraction were analyzed by Western blotting using the antibodies indicated on the right side of the panel.