TABLE 2.
dsDNA and ssRNAs are unable to inhibit TLR3 signaling
| Ligand or expression plasmid (μg/ml)a | Form | Amt (μg/ml) of poly(I-C) used | TLR3 activityb (%) |
|---|---|---|---|
| None | None | 15 | |
| None | 2.5 | 100 (7) | |
| Plasmid A (12.5) | dsDNA | 2.5 | 114 (9) |
| Plasmid A (25) | dsDNA | 2.5 | 100 (9) |
| Plasmid B (12.5) | dsDNA | 2.5 | 102 (3) |
| Plasmid B (25) | dsDNA | 2.5 | 114 (17) |
| 54-bp DNA (6.6) | dsDNA | 2.5 | 83 (9) |
| 54-bp DNA (17) | dsDNA | 2.5 | 63 (5) |
| Poly(I) (12.5) | ssRNA | 2.5 | 78 (3) |
| Poly(I) (25) | ssRNA | 2.5 | 75 (9) |
| Poly(C) (12.5) | ssRNA 2.5 | 2.5 | 91 (22) |
| Poly(C) (25) | ssRNA 2.5 | 2.5 | 78 (13) |
| Poly(U) (12.5) | ssRNA 2.5 | 2.5 | 110 (9) |
| Poly(U) (25) | ssRNA 2.5 | 2.5 | 109 (4) |
| Poly(I-U) (12.5) | ssRNA | 2.5 | 76 (3) |
| Poly(I-U) (25) | ssRNA | 2.5 | 73 (5) |
a
Plasmid A, 6.5-kb pcDNA-derived plasmid; plasmid B, 9.0-kb pcDNA-derived plasmid; none, no ligand or plasmid added.
b
Data are the means of the results of five sets of experiments. The numbers in parentheses denote 1 standard error.