TABLE 3.
Correlation between oligonucleotide length and TLR3 inhibitory activity
| Length (nt) | Sequence | NF-κβ activitya (%) (SE) |
|---|---|---|
| 19 | CCTTACCCATAATCAACTC | 105 (8) |
| 24 | TTGGCCCTTACCCATAATCAACTC | 86 (15) |
| 25 | GTCGACAAGGGATTGAACCTCGTTC | 103 (8) |
| 29 | CAGTGTTGGCCCTTACCCATAATCAACTC | 58 (6) |
| 30 | TACGTACTTAGATATGTCTTCAAACCATAC | 67 (3) |
| 34 | CATAACAGTGTTGGCCCTTACCCATAATCAACTC | 44 (3) |
| 36 | GGCTTCACTTTCATAGACTGCAACGGTGCCTGAAAG | 38 (1) |
| 39 | ACAAACATAACAGTGTTGGCCCTTACCCATAATCAACTC | 38 (1) |
| 40 | GCTCTAGATTAAGATTTACCGATGTCGCTCACTAAGGACC | 32 (1) |
| 41 | AGTTCTAGACTACGTGACGACCTCCAGGTCAGCCGACATGC | 35 (5) |
| 43 | ACAACTTAAATGTGGTTGGTGCCGATTCCTTTGCTTGGCTTCC | 33 (1) |
| 45 | GAAGAACTGGATATCTTTGCCGCTTCATCTTTAAAAAAATTAGAG | 47 (4) |
| 53 | AGTGCTAGCAGTCATCCAACAGAATCATGTCGATGTCCACTACATGGACAGGC | 38 (4) |
| 54 | CAGTGCTAGCAGTCATCCAACAGAATCATGAATGGAATATTTGCTATTGAGAAG | 36 (2) |
| 56 | AGTGCTAGCAGTCATCCAACAGAATCATGGCCCCCATCACGTACTCCACCTATGGC | 22 (4) |
| 57 | AGTGCTAGCAGTCATCCAACAGAATCATGAGCACCTGGGTGCTGGTAGGCGGAGTCC | 29 (2) |
| 58 | AGTGCTAGCAGTCATCCAACAGAATCATGGCCTCACACCTCCCTTACATCGAAGACGG | 27 (4) |
| 60 | CTCGAGCAGAGGTCTCACACAGAGACAAGCGCATCAGTCAACAGAGATCCCTTGCGCTTC | 35 (1) |
| 75 | GTCGACCACGGTTCTGCTACTTGTTCTTTGTTTTTCACCAACAAAATGGTATGGTTTGAAGACATATCTAAGTAC | 30 (7) |
a
TLR3 activity obtained with poly(I-C) as the ligand was set at 100%.