FIG. 4.

eEF1A2-mediated formation of filopodia is dependent on PI(4,5)P₂. Rat2 cells stably expressing eEF1A2 were transfected with mRFP-FKBP-5-ptase, PM-FRB-CFP, and PLCδ-PH-GFP. (A) Addition of rapamycin (+Rapamycin) depletes the levels of membrane-bound PI(4,5)P₂ and reduces production of filopodia (arrows). (B) Addition of rapamycin, without mRFP-FKBP-5-ptase and PM-FRB-CFP, has no effect on eEF1A2-dependent PI(4,5)P₂ accumulation on the plasma membrane or on development of filopodia (arrows). Scale bars represent 10 μm.