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. 2008 May 12;28(14):4549–4561. doi: 10.1128/MCB.00150-08

FIG. 6.

FIG. 6.

PI4KIIIβ is sufficient to induce production of filopodia. (A) Western blots showing PI4KIIIβ overexpression in Rat2 stable cell lines. (B) Rat2 cells stably overexpressing PI4KIIIβ have greater PI4KIIIβ lipid kinase activity, as seen by an increase in the PI4P product. The TLC is a representative sample of three independent in vitro kinase assays. (C) Left panels, PI4KIIIβ-overexpressing Rat2 stable cells show more and longer filopodia than vector controls. Right panels, quantification of filopodia (n = 134 and 118 for vector stable cells and PI4KIIIβ stable cells, respectively). Filled columns indicate cells with at least 10 filopodia greater than or equal to 3 μm in length. Open columns indicate cells with any number of filopodia less than 3 μm in length. Significance (P < 0.05, Student's t test) is indicated by an asterisk. (D) PI4KIIIβ-overexpressing Rat2 stable cells show more membrane-bound PI(4,5)P2 than control cells. The PI(4,5)P2 localizes to the plasma membrane as well as to parts of filopodia. Scale bars represent 10 μm.