TABLE 2.

Activity of 5S genes in yeast strains with different gene copy numbers, strains at different growth stages, and strains with mutations

Strain (genotype)	Growth stage	Approximate no. of rDNA repeats/cell	No. of 5S genes tallied	Active 5S genes/cell (%)	Approximate no. of active 5S genes/cellc	Mean no. of pols/active gene	SD	95% CI	Approximate no. of pols on 5S genes/celld
NOY886	Log	42b	464	69	29	1.96	0.74	0.08	57
	30 min of rapamycin	42	103	46	19	1.87	0.82	0.24	36
	Post-log	42	122	34	14	1.39	0.59	0.18	19
NOY1051a	Log	143	347	30	43	1.73	0.71	0.14	74
NOY388 (wt SPT4)	Log	177	227	21	37	1.81	0.88	0.25	67
NOY2167 (spt4Δ)	Log	64	234	35	22	1.77	0.79	0.17	39
CY1 (wt LHP1)	Log	190	249	26	49	1.54	0.66	0.13	75
CY4 (lhp1Δ)	Log	140	160	34	48	2.12	0.67	0.14	102

^aThe NOY1051 data include the results for the single nucleolus shown in Fig. 3, as well as additional data representing smaller-rDNA-repeat multimers from this strain, similar to the data used for the other strains shown here. See breakdown in Table S5 in the supplemental material.

^bThe four bold rows show data for four logarithmically growing control strains with different gene copy numbers. These data were used for the graphs in Fig. 4A to C.

^cDerived from rDNA repeat number and estimated percentage of active genes per cell.

^dDerived from approximate number of active 5S genes/cell and mean number of pols/active gene.