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. 2008 May 19;28(14):4445–4458. doi: 10.1128/MCB.00164-08

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Copyright © 2008, American Society for Microbiology

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FIG. 2. — The Rco1 PHD domain affects pob3(L78R), and rco1 suppresses the spt16-11 htz1 and spt16-11 H4(K5R,K12R) synthetic lethalities. (A) Tenfold dilutions of strains DY150 (wild type), DY10398 (rco1), DY12800 [RCO1(ΔPHD)], DY12881 [RCO1(Yng2-PHD)], DY7379 [pob3(L78R)], DY10406 [pob3(L78R) rco1], DY12802 [RCO1(ΔPHD) pob3(L78R)], and DY12883 [pob3(L78R) RCO1(Yng2-PHD)] were plated on complete medium for 4 days at either 20°C or 27°C. (B) Tenfold dilutions of strains DY150 (wild type), DY10398 (rco1), DY7835 (htz1), DY8107 (spt16-11), DY11612 (htz1 rco1), DY11373 (spt16-11 rco1), DY9807 (spt16-11 htz1), and DY11606 (spt16-11 htz1 rco1) were plated on complete medium for 3 days at 30°C. (C) Tenfold dilutions of strains DY11848 (spt16-11), DY11850 (spt16-11 rco1), DY11852 [spt16-11 HHF2(K5, K12R)], and DY11854 [spt16-11 rco1 HHF2(K5, K12R)] were plated on complete medium for 4 days at either 25°C or 30°C.