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RCO1 overexpression is toxic in esa1 and arp4 mutants. Strains DY150 (wild type), DY7560 [esa1(L254P)], DY7558 (esa1-Δ414), and DY5856 (arp4-3) were transformed with the indicated multicopy plasmid, and 10-fold dilutions were plated on selective medium lacking uracil. All plates were incubated for 3 days at the indicated temperature for 2 days, except for the esa1-Δ414 cells at 35°C, which were incubated for 4 days.
