FIG. 6.

Analysis of regulation of β-catenin signaling activity by wild-type and mutant TRβ by luciferase reporters. FH-TRβ, FH-TRβPV, and FH cells were transfected with either the β-catenin-TCF4-responsive TOP-Flash luciferase reporter or a FOP-Flash reporter that harbors mutations in the TCF4 DNA binding sequences, along with the expression vectors pcDNA-hTCF-4 (TCF4) and pcDNA3-β-catenin or the empty vector pcDNA3.1, as indicated in Materials and Methods. Results of three independent experiments for TOP-Flash activity (A) and FOP-Flash activity (B) are shown. Bars are means ± SEM. NS, not significant.