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. 2008 May 12;28(14):4520–4535. doi: 10.1128/MCB.02011-07

FIG. 5.

FIG. 5.

Regulation of RASSF1A self-association. (A) Coimmunoprecipitation (IP) of GFP-RASSF1A and HA-RASSF1A with anti-HA antibodies following TNF-α stimulation in U2OS cells (left panel) or H1299 cells (right panel) as indicated. IB, immunoblotting. (B) Schematic of RASSF1A deletion mutants used in panel C indicating the location of the C1 domain containing a potential zinc finger binding motif. Numbers indicate amino acid locations. (C) Coimmunoprecipitation of GFP-RASSF1A (GFP-1A) and HA-RASSF1A (HA-1A) deletion mutants in U2OS cells using anti-HA antibodies.