FIG. 6.

Targeting strategy to eliminate the expression of both GABPβ1L and GABPβ1S. (A) Schematic of the targeting strategy. In the targeted allele, exons 2 to 6 were replaced with a neomycin (NEO) cassette. Key restriction enzyme sites and probes used in Southern blotting are indicated. The neomycin cassette contains each of the MscI and SpeI sites, and size changes of MscI and SpeI digestion in the WT and targeted alleles are also indicated. (B) Identification of ES cells with correct homologous recombination. Genomic DNA was extracted from ES cells that were electroporated with the targeting construct, digested with either MscI (left) or SpeI (right), and probed with up- or downstream probes as marked in A, respectively.