TABLE 1.
Characteristics of the GTPases selected for the first screen and their effect on pckb-2::gfp reporter activationa
| Phenotype | WormBase IDb | Designation | Subcellular localizationc | Functions related to the ER (reference) |
|---|---|---|---|---|
| No effect | C44C11.1 | RAS-1 | Endomembrane (ER/G) | Intracellular protein transport, ER signal transduction (33) |
| F53G12.1 | RAB-11.1 | TGN recycling/exocytic compartment | Membrane fusion, vesicular trafficking | |
| F54C9.10 | ARL-1 | TGN/G | Membrane fusion and traffic | |
| F53F10.4 | UNC-108 | ER/cis-G | Endocytosis, membrane fusion, vesicular trafficking | |
| W01H2.3 | RAB-37 | Secretory granules | Vesicle exocytosis | |
| ZK792.6 | LET-60 | Endomembrane (ER/G) | Intracellular protein transport, ER signal transduction (33) | |
| C27B7.8 | RAP-1 | G | Membrane fusion, vesicular trafficking | |
| F45E4.1 | ARF-1.1 | G/ERGIC/TGN | Mitotic Golgi fragmentation, vesicular trafficking | |
| Severe developmental defects | C39F7.4 | RAB-1 | ER/cis-G | Intracellular vesicle trafficking |
| W03C9.3 | RAB-7 | ER/LE/endosome | Endosomal traffic, vesicular trafficking | |
| Induction of basal ER stress | ZK180.4 | SAR-1 | ER | ER-to-Golgi protein transport |
| ER stress inhibition | R07G3.1 | CDC-42 | ER/G | Cell polarity, protein trafficking |
| Y32F6B.3 | CRP-1 | TGN/RE | Membrane trafficking, cell polarity (19) |
a
pckb-2::gfp reporter worms were treated with selected GTPase RNAi vectors and exposed or not to TM for 5 hours. The consequence of GTPase silencing on the activation of ckb-2 promoters was measured using a fluorescence microscope, and GTPases were classified according to their effect on this reporter into four categories.
b
ID, identification.
c
G, Golgi apparatus; TGN, trans-Golgi network; ERGIC, ER-Golgi intermediate compartment; LE, late endosomes; RE, recycling endosomes.