FIG. 7.

c-Jun is required for RNA polymerase II recruitment and activity during ccl2 mRNA synthesis. (A) Cells were kept in low-concentration serum as described for Fig. 1. IL-1-induced recruitment of polymerase II was analyzed by ChIP using untreated cells or IL-1-stimulated cells at the indicated time points. wt, wild type. (B) cDNAs derived from cells kept in low-concentration serum as described for Fig. 1 were analyzed for expression of ccl2 pre-mRNA and mature mRNA. The regions of murine ccl2 transcripts that were examined are indicated in the upper panel. Amounts of PCR products were quantitated against a standard curve derived from serially diluted genomic DNA by use of Sybr green.