FIG. 8.

c-Jun mediates histone modifications, histone deacetylase binding, and NF-κB recruitment at the murine ccl2 gene locus. (A) Schematic representation of the three selected genomic regions of the murine ccl2 gene (indicated by white boxes) that were analyzed by PCR. (B) Wild-type (wt) or c-Jun-deficient (−/−) fibroblasts were treated for 2 h with 10 ng/ml IL-1α or were left untreated. After in vivo cross-linking, chromatin was isolated from all cultures and immunoprecipitated with the indicated antibodies. Genomic DNA fragments from the indicated regions of mouse ccl2 were amplified by real-time PCR and amounts of DNA quantitated using a standard curve. Data represent mean amounts of DNA (in nanograms) ± standard errors of the means of the results of at least two independent experiments. The dashed lines indicate nonspecifically bound DNA fragments as determined by the IgG control ChIP. “NF-κB” indicates a primer pair that covers the region around the two NF-κB-binding sites as shown in panel A.