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. 2008 May 5;28(13):4285–4299. doi: 10.1128/MCB.01240-07

FIG. 9.

FIG. 9.

Oncogenic signaling leads to insensitivity to clusterin-mediated inhibition. (A and B) The control (Con)- or clusterin (Clu)-containing HEK293T-CM was mixed with HeLa-CM (1:1), and samples were tested for their abilities to affect Akt phosphorylation in HEK293 and HEK293T cells. (C) The Con- or Clu-containing HEK293T-CM was mixed with HeLa-CM in the presence of the indicated amounts of LY294002 and then tested for their effects on Akt phosphorylation in HEK293T cells. (D) The Con- or Clu-containing HEK293T-CM was mixed (1:1) with HeLa-CM and tested for the ability to affect HeLa-CM-mediated Akt phosphorylation in HEK293-Ras or HEK293-vector cells. (E) Whole-cell lysates of serum-starved HeLa, HEK293, HEK293T, or HEK-Ras and their corresponding CM fractions were tested for Akt-activating activity in NB4 cells.