FIG. 5.

Fbl6 regulates Yan downregulation in vivo. (A) P{GawB}NP0326 flies have strongly diminished levels of fbl6 expression. RT-PCR was performed on cDNA prepared from stage-15 embryos derived from the indicated fly lines. Levels of tubulin transcript were determined as a control for the procedure. (B) The P element of P{GawB}NP0326 flies specifically disrupts fbl6 and not adjacent transcripts. RT-PCR was performed as described for panel A. (C) Yan protein levels are elevated in P{GawB}NP0326 embryos. Embryos were carefully staged to stage 12 and lysed in Laemmli sample buffer. Yan protein levels were determined by Western blotting with a Yan monoclonal antibody, and tubulin protein levels were determined as a loading control. (D) Expression of argos is attenuated in P{GawB}NP0326 embryos. cDNA was prepared from total RNA isolated from differently staged embryos. Levels of argos expression and control tubulin expression were determined by RT-PCR.