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. Author manuscript; available in PMC: 2008 Jul 9.
Published in final edited form as: Oncogene. 2007 Nov 12;27(18):2575–2582. doi: 10.1038/sj.onc.1210919

Figure 3.

Figure 3

HPV-16 E6 oncogene reduces the expression of miR-218. (A) qRT-PCR analysis of miR-218 and SLIT2 in U2OS-NEO, U2OS-16E6, and U2OS-16E7. (B) Expression of HPV-16 E6 and E7, miR-218 and SLIT2 in the 20861 cell line with or without RNAi against HPV-16 E6/E7. (C) qRT-PCR analysis of miR-218 in NOK-NEO, NOK cell line expressing the E6 gene of either the low-risk HPV-6 (NOK-6E6) or high-risk HPV-16 (NOK-16E6). RNU43 served as the endogenous control for miRNAs, while G3PDH served as the endogenous control for E6, E7 and SLIT2.