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. Author manuscript; available in PMC: 2008 Jul 9.
Published in final edited form as: Oncogene. 2007 Nov 12;27(18):2575–2582. doi: 10.1038/sj.onc.1210919

Figure 4.

Figure 4

Expression of LAMB3 is reduced in the presence of miR-218. (A) Northern blot analysis of miR-218 after transfection of a precursor of miR-218 in SiHa and 20861 cell lines. U6 RNA was used as a loading control. (B) qRT-PCR analysis of LAMB3 in SiHa and 20861 cell lines transfected with a pre-miR-218. (C) Western blot analysis of LAMB3 protein in SiHa cells transfected with pre-miR-218. Location of the 40-kDa LAMB3 protein is indicated. G3PDH was used as a control. (D) Expression of LAMB3 mRNA in the 20861 cell line with or without RNAi against HPV-16 E6/E7, and in the U2OS-NEO and U2OS-16E6 cell lines. G3PDH served as the endogenous control for LAMB3.