Table 1.
Primers/probe* | Sequence (5′ → 3′) | Ref. |
---|---|---|
SDBact0338aA18† | ACTCCTACGGGAGGCAGC | 12 |
SDBact0515aA19 | GTGCCSGCMGCCGCGGTAA | 13‡ |
SDBact0515aS19 | TTACCGCGGCMGCSGGCAC | 13‡ |
SDBact1371aS20 | AGGCCCGGGAACGTATTCAC | 14 |
SDBact1525aS17 | AAGGAGGTGATCCAGCC | 15‡ |
SDBact1529aS17 | YAKAAAGGAGGTGWTCC | This study |
SGTrep0008aA20 | AGAGTTTGATCMTGGCTCAG | 9 |
SGStrp1264aS22§ | AGAGATTAGCTTGCCGTCACCG | This study |
Primers and probes are named according to Oligonucleotide Probe Database convention (16). The names incorporate the 5′ terminal position (E. coli 16S rRNA numbering convention) and primer length in nucleotides.
† Orientation indicates PCR primer sequence. Reverse complement sequence used for in situ probing.
‡ Modified from indicated reference.
§ Streptococcus-specific probe.