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SpoIIIE-GFP localization (B and C, green) in KP542 sporangia stained with FM 4-64 (A and B, red) and DAPI (C and D, blue) at t3. SpoIIIE initially localizes to the polar septum (arrow 1), then moves with the engulfing membranes (arrow 2) to the cell pole just before membrane fusion (arrow 3), where it remains after fusion is complete (arrow 4) until the protein delocalizes (arrow 5). (White bar in A is 2 μm.) A larger field can be seen in supplemental Fig. 6 at www.pnas.org.
