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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Exp Neurol. 2008 Mar 10;211(2):529–538. doi: 10.1016/j.expneurol.2008.02.023

Fig. 2.

Fig. 2

VP protects H32 hypothalamic cells from serum deprivation-induced apoptotic cell death. FACS analysis of the percentage of H32 hypothalamic cells undergoing early (annexin V +/7-AAD− -labeled cells) and late apoptosis (annexin V +/7-AAD+-labeled cells) after 6 or 24 h incubation in 20% serum or serum-free medium without or with 10nM VP. The results were plotted as fluorescence intensity of annexin V as a function of fluorescence intensity of 7-AAD. The numbers in the corner of each square present the percentage of cells for annexin V −/7-AAD−-viable cells (the number in left low corner); annexin V +/7-AAD− early apoptotic cells (the number in right low corner); annexin V +/7-AAD+-lated apoptotic cells (the number in right high corner) and annexin V −/7-AAD+-broken cells (the number in left high corner). Plots are representative of the results in three different experiments.