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. 2008 Apr 18;283(16):10425–10432. doi: 10.1074/jbc.M800729200

FIGURE 2.

FIGURE 2.

OAREKI required for CAR to activate the CYP2B6 promoter. The –1.8 kb promoter, its deletion mutant (–1.8 kb ΔOAREKI), PBREM/–307, or its deletion mutant (PBREM/–307ΔOAREKI) was co-transfected with phRL-SV40 into Ym17 (A and B) or HepG2 (C and D) cells. The cells were treated with Me2SO (DMSO), 250 nm TCPOBOP (TC), 10 nm OA, or TCPOBOP plus OA (OA&TC) and were harvested for measuring luciferase activity. Reporter activity was calculated by dividing firefly luciferase with Renilla luciferase activities (A and C). -Fold activation was calculated by taking control activity (Me2SO) as 1 (B and D). WT, wild type.