TABLE 1.
Yeast strains and plasmids used in this study
|
Strain
|
Relevant genotype
|
Source
|
||||
| W303 background | ||||||
| YKA150 | MATabar1::URA3 | 60 | ||||
| YKA247 | MATabar1::URA3 acm1::KanMX4 | 26 | ||||
| YKA254 | MATaacm1::KanMX4 | This study | ||||
| YKA415 | MATabar1::URA3 cdc23-1 | This study | ||||
| HCY114 | MATacdc14-1 | This study | ||||
| HCY115 | MATaleu2::GAL-HA-CDC14:LEU2 | This study | ||||
| HCY116 | MATaleu2::GAL-HA-CDC14C283S:LEU2 | This study | ||||
| DLY3033 | MATacdc15-2 | Daniel Lew, Duke University | ||||
| RJD2632 | MATacdc28-as1 | Raymond Deshaies, California Institute of Technology | ||||
|
5397
|
MATacdc28-4
|
Steven Reed, Scripps
|
||||
| BY4741 background | ||||||
| YKA170 | MATacdh1::KanMX4 CLB2-3HA:HIS3 | This study | ||||
| YKA226 | MATa3HA-ACM1 | 26 | ||||
| YKA242 | MATabar1::hisG acm1::URA3 CDH1-3FLAG:KanMX4 | This study | ||||
| YKA244 | MATacdh1::KanMX4 | This study | ||||
| YKA245 | MATabar1::URA3 cdh1::KanMX4 3HA-ACM1 | 26 | ||||
| YKA249 | MATabar1::hisG 3HA-BMH1 | 26 | ||||
| YKA253 | MATabar1::hisG acm1::KanMX4 | This study | ||||
| YKA295 | MATabar1::hisG acm1::KanMX4 3FLAG-BMH1 | This study | ||||
|
YKA407
|
MATabar1::hisG acm1::KanMX4
pdr5::URA3
|
This study
|
||||
| Plasmid name | Origin | Marker | Promoter | Expressed protein | ||
| pHLP101 | CEN/ARS | LEU2 | ADH | HA-Acm1 | ||
| pHLP102 | CEN/ARS | LEU2 | ADH | HA-Acm1-5A | ||
| pHLP103 | CEN/ARS | LEU2 | ADH | HA-Acm1-5E | ||
| pHLP107 | CEN/ARS | LEU2 | ADH | 3FLAG-Acm1 | ||
| pHLP109 | CEN/ARS | LEU2 | GAL1 | HA-Acm1 | ||
| pHLP110 | CEN/ARS | LEU2 | GAL1 | HA-Acm1-5A | ||
| pHLP111 | CEN/ARS | LEU2 | GAL1 | HA-Acm1-5E | ||
| pHLP112 | CEN/ARS | URA3 | GAL1 | 3FLAG-Acm1 | ||
| pHLP113 | CEN/ARS | URA3 | GAL1 | 3FLAG-Acm1-5A | ||
| pHLP117 | CEN/ARS | LEU2 | ACM1 | 3HA-Acm1 | ||
| pHLP130 | CEN/ARS | LEU2 | ADH | 3FLAG-Cdh1 | ||
| pHLP135 | CEN/ARS | LEU2 | ACM1 | 3HA-Acm1-S37A | ||
| pHLP136 | CEN/ARS | LEU2 | ACM1 | 3HA-Acm1-S48A | ||
| pHLP137 | CEN/ARS | LEU2 | ACM1 | 3HA-Acm1-S102A | ||
| pHLP138 | CEN/ARS | LEU2 | ACM1 | 3HA-Acm1-S202A | ||
| pHLP139 | CEN/ARS | LEU2 | ACM1 | 3HA-Acm1-T161A | ||
| pHLP150 | CEN/ARS | LEU2 | ACM1 | 3HA-Acm1-S3A | ||
| pHLP169 | CEN/ARS | LEU2 | ACM1 | 3HA-Acm1-5A/T161 | ||
| pHLP183 | CEN/ARS | URA3 | CDC28 | Cdc28 | ||
| pHLP185 | CEN/ARS | LEU2 | GAL1 | HA-Acm1-T161A | ||
| pHLP209 | CEN/ARS | LEU2 | ACM1 | 3HA-Acm1-5A | ||
| pHLP212 | CEN/ARS | LEU2 | GAL1 | 3HA-Acm1 | ||
| pHLP222 | CEN/ARS | LEU2 | ACM1 | 3HA-Acm1-5E | ||
| pHLP231 | CEN/ARS | TRP1 | GAL1 | 3FLAG-Cdh1 | ||
| pHLP232 | CEN/ARS | TRP1 | GAL1 | 3FLAG-Cdh1-11A | ||
| pESCLeu-mycCDC14 | 2 μm | LEU2 | GAL1 | myc-Cdc14 | ||
| pESCTrp-FIN1myc | 2 μm | TRP1 | GAL1 | Fin1-myc | ||