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. 2008 Apr 18;283(16):11024–11037. doi: 10.1074/jbc.M800312200

FIGURE 2.

FIGURE 2.

Intermolecular NOEs observed in the IIAMan-IIBMan complex. Methyl region of 1H(13C-attached, F3)/13C(F2) planes from a three-dimensional 12C-filtered(F1)/13C-separated(F2) NOE-HSQC spectrum displaying NOEs from the methyl protons of Met-103 (top panels), Met-23 (middle panels), and Leu-129 δ1 (bottom panels) of [12CH3-LV]/[1H-MV]/[2H/12C/14N]-IIAMan to protons attached to 13Cof[U-15N/13C]-IIBMan. A, wild-type IIAMan(H10) and IIBMan. B, IIAMan(H10E) and IIBMan. C, location of the two clusters of residues located on helices α1 and α3 of IIBMan that are far apart in the structure but exhibit NOEs to the methyl group of Met-103 in the wild-type sample. The wild-type sample (A) displays NOEs arising from a mixture of two complexes comprising one that is compatible with phosphoryl transfer (productive) and the other where phosphoryl transfer cannot take place (non-productive); the complex of the phosphomimetic IIAMan(H10E) with IIBMan (B) corresponds to a single productive complex.