FIGURE 1.
IP3R induction by dexamethasone. A, signal intensities of labeled complementary RNA hybridized to oligonucleotide probes on Affymetrix arrays for IP3R1 and IP3R2 in WEHI7.2 and S49.A2 lymphoma cells. Cells were treated for the indicated times with ethanol vehicle (white bars) or 1 μm dexamethasone (black bars). Data represent the mean of duplicate samples. B, Northern blot analysis confirming elevation of IP3R1 and IP3R2 mRNA levels in WEHI7.2 cells following treatment with 1 μm dexamethasone (DEX). Results are representative of five experiments. C and D, immunoblots confirming elevation of IP3Rs in WEHI7.2 and S49.A2 cells treated with 1 μm dexamethasone. Results are representative of multiple independent experiments: five for WEHI7.2 and three for S49.A2.