Skip to main content
. 2008 Apr 18;283(16):10385–10395. doi: 10.1074/jbc.M710231200

FIGURE 1.

FIGURE 1.

Endogenous expression of TRPC3 in human hematopoietic cells. Western blotting was performed on lysates from UT-7 and TF-1 Epo-responsive cell lines, from CD34+ cells and from day 10 and 14 BFU-E-derived erythroblasts. Equivalent amounts of protein were loaded in each lane. A, immunoblotting with anti-TRPC3 antibody demonstrated increased expression of TRPC3 in primary human erythroid cells during erythroid differentiation. Blots were probed with anti-actin antibody to compare loading of lanes. Representative results of four experiments are shown. B, densitometry was used to quantitate TRPC3 and actin bands from four experiments of lysates from CD34+ cells and day 10 and 14 BFU-E-derived erythroblasts. The TRPC3/actin ratio was calculated and normalized to CD34+ cells to allow comparison between experiments, and the mean normalized ratio ± S.E. was determined. TRPC3 expression was significantly less in CD34+ cells than in day 10 erythroblasts (p < 0.02). C, immunoblotting with anti-Epo-R antibody demonstrated greatest Epo-R expression in day 10 primary erythroblasts. Representative results of three experiments are shown.