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. 2008 Apr 18;283(16):10385–10395. doi: 10.1074/jbc.M710231200

FIGURE 6.

FIGURE 6.

Association of TRPC3 and TRPC3-F4 with PLCγ or Epo-R. A, PLCγ and V5-TRPC3 or V5-TRPC3-F4 were expressed in HEK 293T cells. Immunoprecipitation (IP) was performed on lysates with anti-PLCγ or anti-V5 antibodies or normal rabbit serum (NRS). Western blotting (WB) was performed after immunoprecipitation with anti-PLCγ or anti-V5 antibodies. Representative results of five experiments are shown. B, immunoprecipitation was performed on lysates from primary human erythroid cells at Phase II day 8 of liquid culture with anti-PLCγ or anti-TRPC3 antibodies or normal rabbit serum. Western blotting of eluates was performed with anti-PLCγ or anti-TRPC3 antibodies. C, Epo-R and V5-TRPC3 or V5-TRPC3-F4 were expressed in HEK 293T cells. Immunoprecipitation was performed on lysates with anti-Epo-R or anti-V5 antibodies or normal rabbit serum. Western blotting was performed with anti-Epo-R or anti-V5 antibodies. Representative results of three experiments are shown. Tx'd, transfected.