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Interaction of TRPC3 with PLCγ SH2 binding site substitutions with PLCγ. PLCγ and V5-TRPC3, V5-TRPC3-F4, or V5-TRPC3-Y226F were expressed in HEK 293T cells. Immunoprecipitation (IP) was performed on lysates with anti-PLCγ or anti-V5 antibodies, followed by Western blotting (WB). Representative results of six experiments are shown. Tx'd, transfected.
