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Association of TRPC3 IP3R binding mutants with IP3R. IP3R type II and V5-TRPC3, V5-TRPC3-DEL, or V5-TRPC3-SUB were expressed in HEK 293T cells. Immunoprecipitation (IP) was performed on lysates with anti-V5 or anti-IP3R antibodies or normal rabbit serum (NRS). Western blotting (WB) of precipitated protein was performed with anti-IP3R or anti-V5 antibodies. Representative results of three experiments are shown.
