TABLE 1.
Epo stimulation of [Ca2+]i in HEK 293T cells transfected with Epo-R, TRPC3, or TRPC6
Fura Red-loaded HEK 293T cells transfected with BFP-TRPC3 or BFP-TRPC6 and Epo-R were treated with 40 units/ml Epo. [Ca2+]i (mean ± S.E. in nm) was measured at base line, and the peak measurement was obtained after monitoring over 20 min of Epo stimulation (40 units/ml). Percentage increase (% Inc) above base line (mean ± S.E.) = peak [Ca2+]i/base-line [Ca2+]i × 100%, minus 100% (base line). n, number of individual cells studied.
|
Transfection
|
Stimulation
|
[Ca2+]i
|
% Inc
|
n
|
||
|---|---|---|---|---|---|---|
| Base line | Peak | |||||
| nm | % | |||||
| BFP-TRPC3 + Epo-R | PBS | 37 ± 3 | 51 ± 8 | 39 ± 24a | 8 | |
| BFP-TRPC3 | Epo | 33 ± 2 | 60 ± 5 | 82 ± 11a | 22 | |
| BFP-TRPC6 | Epo | 33 ± 2 | 56 ± 4 | 72 ± 10a | 31 | |
| Epo-R | Epo | 32 ± 2 | 65 ± 4 | 107 ± 12a | 18 | |
| BFP-TRPC3 + Epo-R | Epo | 35 ± 1 | 116 ± 3 | 242 ± 10 | 45 | |
| BFP-TRPC6 + Epo-R | Epo | 32 ± 1 | 67 ± 3 | 125 ± 14a | 41 | |
a
A significant difference from Epo-stimulated cells expressing Epo-R and TRPC3 (p < 0.001).