TABLE 2.
Inhibition of Epo-stimulated [Ca2+]i increase through TRPC3 by siRNAi targeted to PLCγ
HEK 293T cells were transfected with BFP-TRPC3 and Epo-R, with siRNA targeted to PLCγ or nonspecific control siRNA. Fura Red-loaded cells were treated with 40 units/ml Epo. [Ca2+]i (mean ± S.E. in nm) was measured at base line and by monitoring over 20 min after Epo stimulation. Percentage increase above base line (% Inc) (mean ± S.E.) = peak [Ca2+]i/base-line [Ca2+]i × 100%, minus 100% (base line). n, number of individual cells studied.
|
RNA interference
|
Stimulation
|
[Ca2+]i
|
% Inc
|
n
|
||
|---|---|---|---|---|---|---|
| Base line | Peak | |||||
| nm | % | |||||
| Control | PBS | 37 ± 2 | 55 ± 4 | 51 ± 10a | 11 | |
| Epo | 34 ± 1 | 122 ± 4 | 267 ± 15 | 19 | ||
| PLCγ 1 | PBS | 34 ± 3 | 57 ± 5 | 68 ± 6a | 10 | |
| Epo | 37 ± 1 | 92 ± 3 | 154 ± 7a | 21 | ||
a
A significant difference from Epo-stimulated cells expressing nonspecific control siRNA (p < 0.0001).