TABLE 4.
Epo activation of TRPC3 IP3R binding mutants
HEK 293T cells were transfected with BFP-TRPC3, BFP-TRPC3-DEL, BFP-TRPC3-SUB, or empty pQBI50 vector and Epo-R. Fura Red-loaded cells were treated with 40 units/ml Epo. [Ca2+]i (mean ± S.E. in nm) was measured at base line and by monitoring over 20 min of Epo stimulation. Percentage increase (% Inc) above base line (mean ± S.E.) = peak [Ca2+]i/base-line [Ca2+]i × 100%, minus 100% (base line). n, number of individual cells studied.
|
TRPC3
|
Stimulation
|
[Ca2+]i
|
% Inc
|
n
|
||
|---|---|---|---|---|---|---|
| Base line | Peak | |||||
| nm | % | |||||
| Wild type | PBS | 40 ± 1 | 62 ± 2 | 54 ± 5a | 11 | |
| Epo | 35 ± 1 | 128 ± 4 | 266 ± 12 | 20 | ||
| TRPC3-DEL | Epo | 38 ± 1 | 86 ± 2 | 127 ± 5a | 27 | |
| TRPC3-SUB | Epo | 33 ± 1 | 83 ± 3 | 150 ± 6a | 21 | |
| Empty vector | Epo | 33 ± 2 | 77 ± 5 | 136 ± 15a | 15 | |